sureprint g3 human gene expression 8 × 60 k microarray Search Results


sureprint g3 human gene expression 8 × 60 k microarray  (Arraystar inc)
90
Arraystar inc sureprint g3 human gene expression 8 × 60 k microarray
Sureprint G3 Human Gene Expression 8 × 60 K Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sureprint g3 human gene expression 8 × 60 k microarray/product/Arraystar inc
Average 90 stars, based on 1 article reviews
sureprint g3 human gene expression 8 × 60 k microarray - by Bioz Stars, 2026-03
90/100 stars
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sureprint g3 human gene expression 8 × 60k microarray  (Welgene Biotech)
90
Welgene Biotech sureprint g3 human gene expression 8 × 60k microarray
Effect of specificity protein (Sp)4 on ANGPTL4 and GBM. ( A ) The protein expression of Sp1, 2, 3, and 4 in normal brain tissue and a brain tumor was validated by Western blotting. Normal brain tissue lysate: GTX27918 (Genetex); Brain tumor tissue: SF268 (Human Glioblastoma, Origene). ( B ) Left panel: heatmap of Sp4-regulated cell movement-related genes. Right panel: The results of <t>microarray</t> analysis after Ingenuity Pathway Analysis (IPA)-mediated function annotation. ( C ) After Sp4 knockdown for 3 days, the protein expression of ANGPTL4 and Sp4 was validated by Western blotting. ( D ) Sp4-regulated genes in which Sp4 associates with the promoter region revealed by chromatin immunoprecipitation coupled with sequencing (ChIP-Seq). ( E ) The defined promoter region of ANGPTL4 and the binding regions of Sp4 determined by ChIP-seq. ( F ) After transfection with the indicated plasmid and pGL2-ANGPTL4 promoter for 2 days, the promoter activity of ANGPTL4 were measured by a reporter assay. Data were expressed as the relative luciferase activity mean ± SEM. (*** p < 0.001). ( G ) After Sp4 overexpression for 48 h, the protein expression was validated by Western blotting. The experiments were performed three times independently, and quantitative results were expressed as the mean ± SEM (* p < 0.05).
Sureprint G3 Human Gene Expression 8 × 60k Microarray, supplied by Welgene Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sureprint g3 human gene expression 8 × 60k microarray/product/Welgene Biotech
Average 90 stars, based on 1 article reviews
sureprint g3 human gene expression 8 × 60k microarray - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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Effect of specificity protein (Sp)4 on ANGPTL4 and GBM. ( A ) The protein expression of Sp1, 2, 3, and 4 in normal brain tissue and a brain tumor was validated by Western blotting. Normal brain tissue lysate: GTX27918 (Genetex); Brain tumor tissue: SF268 (Human Glioblastoma, Origene). ( B ) Left panel: heatmap of Sp4-regulated cell movement-related genes. Right panel: The results of microarray analysis after Ingenuity Pathway Analysis (IPA)-mediated function annotation. ( C ) After Sp4 knockdown for 3 days, the protein expression of ANGPTL4 and Sp4 was validated by Western blotting. ( D ) Sp4-regulated genes in which Sp4 associates with the promoter region revealed by chromatin immunoprecipitation coupled with sequencing (ChIP-Seq). ( E ) The defined promoter region of ANGPTL4 and the binding regions of Sp4 determined by ChIP-seq. ( F ) After transfection with the indicated plasmid and pGL2-ANGPTL4 promoter for 2 days, the promoter activity of ANGPTL4 were measured by a reporter assay. Data were expressed as the relative luciferase activity mean ± SEM. (*** p < 0.001). ( G ) After Sp4 overexpression for 48 h, the protein expression was validated by Western blotting. The experiments were performed three times independently, and quantitative results were expressed as the mean ± SEM (* p < 0.05).

Journal: International Journal of Molecular Sciences

Article Title: ANGPTL4 Induces TMZ Resistance of Glioblastoma by Promoting Cancer Stemness Enrichment via the EGFR/AKT/4E-BP1 Cascade

doi: 10.3390/ijms20225625

Figure Lengend Snippet: Effect of specificity protein (Sp)4 on ANGPTL4 and GBM. ( A ) The protein expression of Sp1, 2, 3, and 4 in normal brain tissue and a brain tumor was validated by Western blotting. Normal brain tissue lysate: GTX27918 (Genetex); Brain tumor tissue: SF268 (Human Glioblastoma, Origene). ( B ) Left panel: heatmap of Sp4-regulated cell movement-related genes. Right panel: The results of microarray analysis after Ingenuity Pathway Analysis (IPA)-mediated function annotation. ( C ) After Sp4 knockdown for 3 days, the protein expression of ANGPTL4 and Sp4 was validated by Western blotting. ( D ) Sp4-regulated genes in which Sp4 associates with the promoter region revealed by chromatin immunoprecipitation coupled with sequencing (ChIP-Seq). ( E ) The defined promoter region of ANGPTL4 and the binding regions of Sp4 determined by ChIP-seq. ( F ) After transfection with the indicated plasmid and pGL2-ANGPTL4 promoter for 2 days, the promoter activity of ANGPTL4 were measured by a reporter assay. Data were expressed as the relative luciferase activity mean ± SEM. (*** p < 0.001). ( G ) After Sp4 overexpression for 48 h, the protein expression was validated by Western blotting. The experiments were performed three times independently, and quantitative results were expressed as the mean ± SEM (* p < 0.05).

Article Snippet: Total RNA was extracted using TRIzol reagent (15596026, Thermo Fisher Scientific) from U87MG cells following control or Sp4 siRNA knockdown for 48 h. Gene expression analysis was performed using a SurePrint G3 Human Gene Expression 8 × 60K Microarray by Welgene Biotech Co., Ltd. (Taipei, Taiwan).

Techniques: Expressing, Western Blot, Microarray, Knockdown, Chromatin Immunoprecipitation, Sequencing, ChIP-sequencing, Binding Assay, Transfection, Plasmid Preparation, Activity Assay, Reporter Assay, Luciferase, Over Expression